Mouse Bcl2 Monoclonal antibody, PBS Only

Bcl2 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5B9

Cat no : 66799-1-PBS

Synonyms

AW986256, B cell leukemia/lymphoma 2, Bcl2, C430015F12Rik, D630044D05Rik, D830018M01Rik

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Validation Data Gallery

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  WB analysis using 66799-1-Ig (same clone as 66799-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66799-1-Ig (same clone as 66799-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66799-1-Ig (same clone as 66799-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 66799-1-Ig (same clone as 66799-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (66799-1-Ig, Clone: 1B5B9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66799-1-PBS targets Bcl2 in WB, IF, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Bcl2 fusion protein Ag24261
• Full Name: B-cell leukemia/lymphoma 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: NM-009741
• Gene Symbol: Bcl2
• Gene ID (NCBI): 12043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.