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BUB3 Recombinant antibody, PBS Only

BUB3 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240087D9

Cat no : 83266-5-PBS

Synonyms

BUB3, BUB3L, hBUB3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BUB3 Recombinant antibody (83266-5-RR)

    WB analysis using 83266-5-RR (same clone as 83266-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83266-5-RR (BUB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BUB3 Recombinant antibody (83266-5-RR)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BUB3 Recombinant antibody (83266-5-RR)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • Affinity experiments of 83266-5-RR

    Affinity experiments of 83266-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83266-5-RR against Human BUB3 were performed. The affinity constant is 0.228 nM.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83266-5-PBS is BUB3 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BUB3 fusion protein Ag25608
Full Name budding uninhibited by benzimidazoles 3 homolog (yeast)
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC005138
Gene Symbol BUB3
Gene ID (NCBI) 9184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.