BUB1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

BUB1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13330-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of MKN-45 using 13330-1-AP (same clone as 13330-1-PBS)

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  WB analysis using 13330-1-AP (same clone as 13330-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13330-1-AP (same clone as 13330-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 13330-1-AP (same clone as 13330-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  IF Staining of IMR-90 cells using 13330-1-AP (same clone as 13330-1-PBS)

  IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 13330-1-AP (same clone as 13330-1-PBS)

  Immunofluorescent analysis of MDCK cells using 13330-1-AP (BUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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Product Information

13330-1-PBS targets BUB1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BUB1 fusion protein Ag4138
• Full Name: budding uninhibited by benzimidazoles 1 homolog (yeast)
• Calculated Molecular Weight: 1085 aa, 122 kDa
• Observed Molecular Weight: 45 kDa, 120 kDa
• GenBank Accession Number: BC028201
• Gene Symbol: BUB1
• Gene ID (NCBI): 699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).