|Positive WB detected in||K-562 cells, HeLa cells|
|Positive IF detected in||IMR-90 cells, MDCK cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13330-1-AP targets BUB1 in WB, IHC, IF, ELISA applications and shows reactivity with human, canine samples.
|Tested Reactivity||human, canine|
|Cited Reactivity||human, Carassius auratus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BUB1 fusion protein Ag4138|
|Full Name||budding uninhibited by benzimidazoles 1 homolog (yeast)|
|Calculated molecular weight||1085 aa, 122 kDa|
|Observed molecular weight||45 kDa, 120 kDa|
|GenBank accession number||BC028201|
|Gene ID (NCBI)||699|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).
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Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma