BTN2A1 Polyclonal antibody, PBS Only

BTN2A1 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20596-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of Jurkat using 20596-1-AP (same clone as 20596-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20596-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 20596-1-AP (same clone as 20596-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20596-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 20596-1-AP (same clone as 20596-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20596-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 20596-1-AP (same clone as 20596-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20596-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 20596-1-AP (same clone as 20596-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20596-1-PBS in a different storage buffer formulation.

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Product Information

20596-1-PBS targets BTN2A1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BTN2A1 fusion protein Ag14545
• Full Name: butyrophilin, subfamily 2, member A1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC016661
• Gene Symbol: BTN2A1
• Gene ID (NCBI): 11120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BTN2A1, also named as BT2.1, BTF1 is a 527 amino acid protein, which belongs to the immunoglobulin superfamily. BTN2A1 is highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas and moderate expression was seen in lung, liver and kidney. The calcualted molecular weight of BTN2A1 is 59 kDa, but glycosylation of BTN2A1 is about 69 kDa. (PMID: 17785817)