BTN2A1 Polyclonal antibody

BTN2A1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20596-1-AP

Synonyms

BK14H9.1, BT2.1, BTF1, BTN2A1

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Validation Data Gallery

• 

  WB analysis of Jurkat using 20596-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 20596-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 20596-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20596-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20596-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, MCF-7 cells, mouse spleen tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20596-1-AP is BTN2A1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BTN2A1 fusion protein Ag14545
• Full Name: butyrophilin, subfamily 2, member A1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC016661
• Gene Symbol: BTN2A1
• Gene ID (NCBI): 11120
• RRID: AB_10693536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTN2A1, also named as BT2.1, BTF1 is a 527 amino acid protein, which belongs to the immunoglobulin superfamily. BTN2A1 is highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas and moderate expression was seen in lung, liver and kidney. The calcualted molecular weight of BTN2A1 is 59 kDa, but glycosylation of BTN2A1 is about 69 kDa. (PMID: 17785817)