BTC Monoclonal antibody, PBS Only

BTC Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H5E8

Cat no : 66683-1-PBS

Synonyms

betacellulin, BTC, Probetacellulin

---

Validation Data Gallery

• 

  WB analysis using 66683-1-Ig (same clone as 66683-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 66683-1-Ig (same clone as 66683-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66683-1-Ig (same clone as 66683-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDA-MB-231 using 66683-1-Ig (same clone as 66683-1-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of T-47D using 66683-1-Ig (same clone as 66683-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66683-1-Ig (same clone as 66683-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66683-1-PBS targets BTC in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BTC fusion protein Ag26724
• Full Name: betacellulin
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC011618
• Gene Symbol: BTC
• Gene ID (NCBI): 685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, binds and activates ErbB1 and ErbB4 homodimers. BTC is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. BTC was expressed in tumors and involved in tumor growth progression.