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BST2 Recombinant antibody, PBS Only

BST2 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7J24

Cat no : 82733-7-PBS

Synonyms

Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BST2 Recombinant antibody (82733-7-RR)

    WB analysis of HeLa using 82733-7-RR (same clone as 82733-7-PBS)

    WB result of BST2 antibody (82733-7-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BST2 transfected HeLa cells. This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BST2 Recombinant antibody (82733-7-RR)

    WB analysis using 82733-7-RR (same clone as 82733-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82733-7-RR (BST2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BST2 Recombinant antibody (82733-7-RR)

    IF Staining of HepG2 using 82733-7-RR (same clone as 82733-7-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BST2 antibody (82733-7-RR, Clone: 7J24 ) at dilution of 1:125 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82733-7-PBS targets BST2 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BST2 fusion protein Ag4430
Full Name bone marrow stromal cell antigen 2
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 30-36 kDa
GenBank Accession NumberBC033873
Gene Symbol BST2
Gene ID (NCBI) 684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).