BST2 Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

BST2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7J24

Cat no : 82733-7-PBS

Synonyms

CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2

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Validation Data Gallery

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  WB analysis using 82733-7-RR (same clone as 82733-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82733-7-RR (BST2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82733-7-RR (same clone as 82733-7-PBS)

  WB result of BST2 antibody (82733-7-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BST2 transfected HeLa cells. This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82733-7-RR (same clone as 82733-7-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BST2 antibody (82733-7-RR, Clone: 7J24 ) at dilution of 1:125 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 82733-7-RR (same clone as 82733-7-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug BST2 Recombinant antibody (82733-7-RR, Clone:7J24) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82733-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82733-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82733-7-RR against Human BST2 were performed. The affinity constant is below 1 pM.

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Product Information

82733-7-PBS targets BST2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BST2 fusion protein Ag4430
• Full Name: bone marrow stromal cell antigen 2
• Calculated Molecular Weight: 180 aa, 20 kDa
• Observed Molecular Weight: 30-36 kDa
• GenBank Accession Number: BC033873
• Gene Symbol: BST2
• Gene ID (NCBI): 684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).