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  • Featured Product
  • KD/KO Validated

BST2 Recombinant antibody

BST2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

7J24

Cat no : 82733-7-RR

Synonyms

CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BST2 Recombinant antibody (82733-7-RR)

    WB analysis using 82733-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82733-7-RR (BST2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BST2 Recombinant antibody (82733-7-RR)

    WB analysis of HeLa using 82733-7-RR

    WB result of BST2 antibody (82733-7-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BST2 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BST2 Recombinant antibody (82733-7-RR)

    IF Staining of HepG2 using 82733-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BST2 antibody (82733-7-RR, Clone: 7J24 ) at dilution of 1:125 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using BST2 Recombinant antibody (82733-7-RR)

    FC experiment of HepG2 using 82733-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug BST2 Recombinant antibody (82733-7-RR, Clone:7J24) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82733-7-RR

    Affinity and Kinetic Characterization of 82733-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82733-7-RR against Human BST2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82733-7-RR targets BST2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BST2 fusion protein Ag4430
Full Name bone marrow stromal cell antigen 2
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 30-36 kDa
GenBank Accession NumberBC033873
Gene Symbol BST2
Gene ID (NCBI) 684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

Protocols

Product Specific Protocols
WB protocol for BST2 antibody 82733-7-RRDownload protocol
IF protocol for BST2 antibody 82733-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols