CoraLite® Plus 488-conjugated BSEP Monoclonal antibody

BSEP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C11D5

Cat no : CL488-67512

Synonyms

ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67512

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 BSEP antibody (CL488-67512, Clone: 3C11D5 ) at dilution of 1:200. Blue (DAPI).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67512 targets BSEP in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BSEP fusion protein Ag29135
• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 11
• Calculated Molecular Weight: 146 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: NM_003742
• Gene Symbol: BSEP
• Gene ID (NCBI): 8647
• RRID: AB_2919487
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ABCB11, also named as BSEP, belongs to the ABC transporter superfamily. ABCB11 involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Mutation of ABCB11 will cause the progressive familial intrahepatic cholestasis type 2 (PFIC2) and the benign recurrent intrahepatic cholestasis type 2 (BRIC2). Genetic variations in ABCB11 may play a role in drug-induced cholestasis causing liver damage.