CoraLite® Plus 488-conjugated BRE Monoclonal antibody

BRE Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E5A5

Cat no : CL488-66356

Synonyms

BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-66356

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 BRE antibody (CL488-66356, Clone: 2E5A5 ) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66356 targets BRE in IF-P applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: BRE fusion protein Ag2256
• Full Name: brain and reproductive organ-expressed (TNFRSF1A modulator)
• Calculated Molecular Weight: 383 aa, 44 kDa
• Observed Molecular Weight: 42-47 kDa
• GenBank Accession Number: BC001251
• Gene Symbol: BRE
• Gene ID (NCBI): 9577
• RRID: AB_2919314
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.