BRD9 Monoclonal antibody

• 

  WB analysis using 68922-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68922-1-Ig (BRD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 68922-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.2 μg BRD9 Monoclonal antibody (68922-1-Ig, Clone:1C10F2, red) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1), and 0.2 μg Mouse IgG1 isotype Control Antibody (66360-1-Ig, Clone: T1F8D3F10, blue) was stained parallely as control. Cells were fixed with 4% PFA .

BRD9 Monoclonal Antibody for WB, FC (Intra), ELISA

Cat No. 68922-1-Ig

Clone No.1C10F2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

UNQ3040/PRO9856, Rhabdomyosarcoma antigen MU-RMS-40.8, LAVS3040, Bromodomain-containing protein 9, BRD 9

---

Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, MDA-MB-231 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68922-1-Ig targets BRD9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BRD9 fusion protein Ag20438
• Full Name: bromodomain containing 9
• Calculated Molecular Weight: 597 aa, 67 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC041590
• Gene Symbol: BRD9
• Gene ID (NCBI): 65980
• RRID: AB_3670453
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9H8M2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD9, also named as MU-RMS-40.8, may play a role in chromatin remodeling and regulation of transcription.