BRCA1 Recombinant antibody, PBS Only

BRCA1 Uni-rAb^TM Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

240285E10

Cat no : 83390-6-PBS

Synonyms

PSCP, IRIS, EC:2.3.2.27, BROVCA1, BRCC1

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Validation Data Gallery

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  WB analysis using 83390-6-RR (same clone as 83390-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83390-6-RR (BRCA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83390-6-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83390-6-RR (same clone as 83390-6-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BRCA1 Recombinant antibody (83390-6-RR, Clone:240285E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83390-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83390-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83390-6-RR against Human BRCA1 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83390-6-PBS is BRCA1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BRCA1 fusion protein Ag17946
• Full Name: breast cancer 1, early onset
• Calculated Molecular Weight: 1863 aa, 208 kDa
• GenBank Accession Number: BC115037
• Gene Symbol: BRCA1
• Gene ID (NCBI): 672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.