BRAP Polyclonal antibody, PBS Only

BRAP Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27431-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of MCF-7 using 27431-1-AP (same clone as 27431-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27431-1-AP (BRAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 27431-1-AP (same clone as 27431-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 27431-1-AP (BRAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 27431-1-AP (same clone as 27431-1-PBS)

  IP result of anti-BRAP (IP:27431-1-AP, 4ug; Detection:27431-1-AP 1:1000) with MCF-7 cells lysate 3200 ug. This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27431-1-AP (same clone as 27431-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27431-1-AP (same clone as 27431-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 27431-1-AP (same clone as 27431-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 27431-1-AP (same clone as 27431-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27431-1-AP (same clone as 27431-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27431-1-AP (same clone as 27431-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

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Product Information

27431-1-PBS targets BRAP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRAP fusion protein Ag26728
• Full Name: BRCA1 associated protein
• Calculated Molecular Weight: 592 aa, 67 kDa
• Observed Molecular Weight: 62-70 kDa, 48 kDa
• GenBank Accession Number: BC136698
• Gene Symbol: BRAP
• Gene ID (NCBI): 8315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.