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BRAP Polyclonal antibody

BRAP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27431-1-AP

Synonyms

BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using BRAP Polyclonal antibody (27431-1-AP)

    WB analysis of MCF-7 using 27431-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27431-1-AP (BRAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using BRAP Polyclonal antibody (27431-1-AP)

    WB analysis of A431 using 27431-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27431-1-AP (BRAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using BRAP Polyclonal antibody (27431-1-AP)

    IP experiment of MCF-7 using 27431-1-AP

    IP result of anti-BRAP (IP:27431-1-AP, 4ug; Detection:27431-1-AP 1:1000) with MCF-7 cells lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of human lung cancer using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of human lung cancer using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of mouse testis using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of mouse testis using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of human breast cancer using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of human breast cancer using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells, A431 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27431-1-AP targets BRAP in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRAP fusion protein Ag26728
Full Name BRCA1 associated protein
Calculated Molecular Weight 592 aa, 67 kDa
Observed Molecular Weight 62-70 kDa, 48 kDa
GenBank Accession NumberBC136698
Gene Symbol BRAP
Gene ID (NCBI) 8315
RRIDAB_2880869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BRAP antibody 27431-1-APDownload protocol
IHC protocol for BRAP antibody 27431-1-APDownload protocol
IP protocol for BRAP antibody 27431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols