CoraLite®594-conjugated BORIS Monoclonal antibody

BORIS Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B12E2

Cat no : CL594-60079

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, dJ579F20.2, Zinc finger protein CTCF T

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Validation Data Gallery

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  IF Staining of HeLa using CL594-60079

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 BORIS antibody (CL594-60079, Clone: 2B12E2 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60079 targets BORIS in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BORIS fusion protein Ag1003
• Full Name: CCCTC-binding factor (zinc finger protein)-like
• Calculated Molecular Weight: 82 kDa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC130486
• Gene Symbol: BORIS/CTCFL
• Gene ID (NCBI): 140690
• RRID: AB_2883418
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.