BORIS Monoclonal antibody, PBS Only

• 

  WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SMMC-7721 using 60079-1-Ig (same clone as 60079-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BORIS antibody (60079-1-Ig, Clone: 2B12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

BORIS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 60079-1-PBS

Clone No.2B12E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, Zinc finger protein CTCF T

---

Product Information

60079-1-PBS targets BORIS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BORIS fusion protein Ag1003
• Full Name: CCCTC-binding factor (zinc finger protein)-like
• Calculated Molecular Weight: 82 kDa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC130486
• Gene Symbol: BORIS/CTCFL
• Gene ID (NCBI): 140690
• RRID: AB_1211604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NI51
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.