NeutraKine® BMP-4 Monoclonal antibody

NeutraKine® BMP-4 Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

3B5A7

Cat no : 69015-1-Ig

Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, NeutraKine® BMP-4, ZYME

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Validation Data Gallery

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  Neutralization experiment of NeutraKine® BMP-4 using 69015-1-Ig

  Recombinant human BMP-4 (Cat.NO. HZ-1045) induces alkaline phosphatase production in the ATDC-5 cell line (using pNPP as chromogenic substrate for detection) in a dose dependent manner (blue curve, bottom X-left Y). The activity of human BMP-4 (200 ng/mL HZ-1045) is neutralized by mouse anti-human BMP-4 antibody 69015-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 3-10 μg/mL.

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Neutralization

Product Information

69015-1-Ig targets NeutraKine® BMP-4 in ELISA, Neutralization applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine BMP-4 protein HZ-1045
• Full Name: bone morphogenetic protein 4
• Gene Symbol: BMP4
• Gene ID (NCBI): 652
• RRID: AB_2918836
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

BMP4, also named as BMP2B and DVR4, belongs to the TGF-beta family. BMPs have been shown to promote astroglial differentiation both in vitro and in vivo. BMP4 have been shown to be components of serum and are thought to be responsible for this differentiation. BMP4 are expressed mainly in neurons, with minimal expression detected in astrocytes and oligodendrocytes.

This antibody can be used to neutralize the bioactivity of BMP-4.