BMI1 Monoclonal antibody

• Featured Product
• KD/KO Validated

BMI1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

CloneNo.

2E10A11

Cat no : 66161-1-Ig

Synonyms

RNF51, Polycomb group RING finger protein 4, Polycomb complex protein BMI-1, PCGF4, 2E10A11

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Validation Data Gallery

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  WB analysis of PC-12 using 66161-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 66161-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 66161-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 66161-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66161-1-Ig

  IP result of anti-BMI1 (IP:66161-1-Ig, 3ug; Detection:66161-1-Ig 1:1000) with HEK-293 cells lysate 1800ug.

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  IHC staining of human lymphoma using 66161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 40x lens.

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  IHC staining of human breast cancer using 66161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66161-1-Ig (BMI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66161-1-Ig (BMI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 10x lens.

• 

  IF Staining of COS-7 using 66161-1-Ig

  Immunofluorescent analysis of COS-7 cells using 66161-1-Ig (BMI1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  IF Staining of HEK-293 using 66161-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BMI1 antibody (66161-1-Ig, Clone: 2E10A11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-12 cells, HT-1080 cells, U2OS cells, U-937 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells, COS-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

66161-1-Ig targets BMI1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BMI1 fusion protein Ag21284
• Full Name: BMI1 polycomb ring finger oncogene
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37-43 kDa
• GenBank Accession Number: BC011652
• Gene Symbol: BMI1
• Gene ID (NCBI): 648
• RRID: AB_2881557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.

Publications

Species	Application	Title
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
human	WB	Autophagy; Inhibition of BMI1 induces autophagy-mediated necroptosis.; Authors - Anindya Dey ; KD Validated
mouse	WB	Clin Transl Med; Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.; Authors - Haiyun Chen
mouse	WB	J Bone Miner Res; Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription; Authors - Qiuyi Wang
human	WB	Aging (Albany NY); Identification of a mesenchymal-related signature associated with clinical prognosis in glioma.; Authors - Zhengwei Zhang
human,mouse	WB,IHC	Mol Cancer Ther; Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.; Authors - Anindya Dey