BIN1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

BIN1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14647-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 14647-1-AP (same clone as 14647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 14647-1-AP (same clone as 14647-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  IP result of anti-BIN1 (IP:14647-1-AP, 4ug; Detection:14647-1-AP 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

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  IHC staining of human osteosarcoma using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

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  IHC staining of human osteosarcoma using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

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Product Information

14647-1-PBS targets BIN1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BIN1 fusion protein Ag6240
• Full Name: bridging integrator 1
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 50-65 kDa
• GenBank Accession Number: BC004101
• Gene Symbol: BIN1
• Gene ID (NCBI): 274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).