BIN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BIN1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 14647-1-AP

Synonyms

Box-dependent myc-interacting protein 1, AMPHL, Amphiphysin-like protein, Amphiphysin like protein, Amphiphysin II

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Validation Data Gallery

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  WB analysis using 14647-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14647-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14647-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14647-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14647-1-AP

  IP result of anti-BIN1 (IP:14647-1-AP, 4ug; Detection:14647-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of mouse skeletal muscle using 14647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 14647-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse brain using 14647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 14647-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse brain using 14647-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14647-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse skeletal muscle tissue, mouse brain tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human osteosarcoma tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

14647-1-AP targets BIN1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BIN1 fusion protein Ag6240
• Full Name: bridging integrator 1
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 50-65 kDa
• GenBank Accession Number: BC004101
• Gene Symbol: BIN1
• Gene ID (NCBI): 274
• RRID: AB_2243396
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).

Publications

Species	Application	Title
human,mouse	WB	Mol Neurodegener; BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia.; Authors - Ari Sudwarts
human	IHC	Int J Cancer; Low expression of Bin1, along with high expression of IDO in tumor tissue and draining lymph nodes, are predictors of poor prognosis for esophageal squamous cell cancer patients.; Authors - Yunlong Jia
human	WB	Mol Biol Cell; Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.; Authors - Giridharan Sai Srinivas Panapakkam SS ; KD Validated
human	WB	Toxicol Appl Pharmacol; Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.; Authors - Azubuike Peter Ebokaiwe
pig	IHC	J Neuropathol Exp Neurol; Combining Hypothermia and Oleuropein Subacutely Protects Subcortical White Matter in a Swine Model of Neonatal Hypoxic-Ischemic Encephalopathy.; Authors - Jennifer K Lee
pig	IHC	J Comp Neurol; Fractional anisotropy from diffusion tensor imaging correlates with acute astrocyte and myelin swelling in neonatal swine models of excitotoxic and hypoxic-ischemic brain injury.; Authors - Jennifer K Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CaX (Verified Customer) (10-24-2023)

An excellent Ab to probe Bin1 at the right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

BIN1 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:14647-1-AP)

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