BID Recombinant monoclonal antibody

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  WB analysis using 84944-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84944-4-RR (BID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84944-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug BID Recombinant antibody (84944-4-RR, Clone:242460F1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  FC experiment of MCF-7 using 84944-4-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BID Recombinant antibody (84944-4-RR, Clone:242460F1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

BID Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84944-4-RR

Clone No.242460F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

p22 BID, 242460F1, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BH3-interacting domain death agonist p11

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Tested Applications

• Positive WB detected in: Jurkat cells, A431 cells, A549 cells, HEK-293 cells, L-929 cells
• Positive FC (Intra) detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84944-4-RR targets BID in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: BH3 interacting domain death agonist
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC009197
• Gene Symbol: BID
• Gene ID (NCBI): 637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P55957
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)