BCS1L Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

BCS1L Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G6H1

Cat no : 60212-1-PBS

Synonyms

h-BCS1, hBCS1, BCS1-like protein, BCS1

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Validation Data Gallery

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  WB analysis of HEK-293 using 60212-1-Ig (same clone as 60212-1-PBS)

  WB result of BCS1L antibody (60212-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCS1L transfected HEK-293 cells. This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60212-1-Ig (same clone as 60212-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60212-1-Ig (BCS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60212-1-Ig (same clone as 60212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60212-1-Ig (same clone as 60212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60212-1-Ig (same clone as 60212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60212-1-Ig (same clone as 60212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60212-1-Ig (same clone as 60212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60212-1-Ig (same clone as 60212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60212-1-Ig (same clone as 60212-1-PBS)

  Immunofluorescent analysis of () fixed MCF-7 cells using 60212-1-Ig (BCS1L antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50678-1

  Cytometric bead array standard curve of MP50678-1, BCS1L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60212-1-PBS. Detection antibody: 60212-2-PBS. Standard:Ag18280. Range: 1.563-200 ng/mL.

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Product Information

60212-1-PBS targets BCS1L as part of a matched antibody pair:

MP50678-1: 60212-1-PBS capture and 60212-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCS1L fusion protein Ag18280
• Full Name: BCS1-like (yeast)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC007500
• Gene Symbol: BCS1L
• Gene ID (NCBI): 617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Human BCS1-like (BCS1L), a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Recently studies indicated that the mutations in this protein may cause the GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome, a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism.