BCS1L Monoclonal antibody

• Featured Product
• KD/KO Validated

BCS1L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G6H1

Cat no : 60212-1-Ig

Synonyms

BCS1L, BJS, FLNMS, GRACILE, h BCS, h BCS1, Hs.6719, Mitochondrial chaperone BCS1, PTD

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Validation Data Gallery

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  WB analysis of HEK-293 using 60212-1-Ig

  WB result of BCS1L antibody (60212-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCS1L transfected HEK-293 cells.

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  WB analysis of COLO 320 using 60212-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60212-1-Ig (BCS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 60212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 60212-1-Ig

  Immunofluorescent analysis of () fixed MCF-7 cells using 60212-1-Ig (BCS1L antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: Colo320 cells, COLO 320 cells, HEK-293 cells
• Positive IHC detected in: human gliomas tissue, human brain tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60212-1-Ig targets BCS1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCS1L fusion protein Ag18280
• Full Name: BCS1-like (yeast)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC007500
• Gene Symbol: BCS1L
• Gene ID (NCBI): 617
• RRID: AB_11042318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human BCS1-like (BCS1L), a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Recently studies indicated that the mutations in this protein may cause the GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome, a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism.