BCR Monoclonal antibody

BCR Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2C2E3

Cat no : 67260-1-Ig

Synonyms

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL

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Validation Data Gallery

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  WB analysis of Jurkat using 67260-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67260-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67260-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of K-562 using 67260-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human BCR (67260-1-Ig, Clone:2C2E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells
• Positive FC (Intra) detected in: K-562 cells
• Positive FC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67260-1-Ig targets BCR in WB, ELISA, FC (Intra) applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCR fusion protein Ag28953
• Full Name: breakpoint cluster region
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC066122
• Gene Symbol: BCR
• Gene ID (NCBI): 613
• RRID: AB_2882531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.