BCLAF1 Monoclonal antibody, PBS Only

BCLAF1 Monoclonal Antibody for WB, IF, FC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6G7

Cat no : 67860-1-PBS

Synonyms

BCLAF1, bK211L9.1, BTF, KIAA0164

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Validation Data Gallery

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  WB analysis using 67860-1-Ig (same clone as 67860-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67860-1-Ig (BCLAF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67860-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67860-1-Ig (same clone as 67860-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCLAF1 antibody (67860-1-Ig, Clone: 1D6G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67860-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67860-1-Ig (same clone as 67860-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human BCLAF1 (67860-1-Ig, Clone:1D6G7) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67860-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67860-1-PBS targets BCLAF1 in WB, IF, FC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCLAF1 fusion protein Ag25210
• Full Name: BCL2-associated transcription factor 1
• Calculated Molecular Weight: 920 aa, 106 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC132780
• Gene Symbol: BCLAF1
• Gene ID (NCBI): 9774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.