BCL6 Recombinant antibody, PBS Only

BCL6 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K6

Cat no : 81754-1-PBS

Synonyms

BCL-6, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B cell lymphoma 6 protein

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Validation Data Gallery

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  WB analysis using 81754-1-RR (same clone as 81754-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81754-1-RR (BCL6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

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  IP experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

  IP result of anti-BCL6 (IP:81754-1-RR, 4ug; Detection:81754-1-RR 1:8000) with Ramos cells lysate 1680 ug. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BCL6 (81754-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81754-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81754-1-RR against Human BCL6 were performed. The affinity constant is 0.157 nM.

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Product Information

81754-1-PBS targets BCL6 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCL6 fusion protein Ag15519
• Full Name: B-cell CLL/lymphoma 6
• Calculated Molecular Weight: 706 aa, 79 kDa
• Observed Molecular Weight: 79-95 kDa
• GenBank Accession Number: BC150184
• Gene Symbol: BCL6
• Gene ID (NCBI): 604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.