BAP31 Recombinant antibody

BAP31 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241978D10

Cat no : 84584-5-RR

Synonyms

BCAP31, BCR-associated protein 31, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, 6C6 AG

---

Validation Data Gallery

• 

  WB analysis using 84584-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84584-5-RR (BAP31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 84584-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAP31 antibody (84584-5-RR, Clone: 241978D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells, MCF-7 cells, THP-1 cells, mouse liver tissue, rat liver tisssue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84584-5-RR targets BAP31 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BAP31 fusion protein Ag1668
• Full Name: B-cell receptor-associated protein 31
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC014323
• Gene Symbol: BAP31
• Gene ID (NCBI): 10134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAP31,aiso know as BCAP31, is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein, and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.