BBS6 Polyclonal antibody, PBS Only

BBS6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13078-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse testis using 13078-1-AP (same clone as 13078-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13078-1-AP (BBS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13078-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 13078-1-AP (same clone as 13078-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13078-1-AP (BBS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13078-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 13078-1-AP (same clone as 13078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13078-1-AP (BBS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13078-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 13078-1-AP (same clone as 13078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13078-1-AP (BBS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13078-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 13078-1-AP (same clone as 13078-1-PBS)

  IF result (cytoplasm and the base of cilia stain) of anti-BBS6 (13078-1-AP; 1:50) with hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 13078-1-PBS in a different storage buffer formulation.

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Product Information

13078-1-PBS targets BBS6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS6 fusion protein Ag3785
• Full Name: McKusick-Kaufman syndrome
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC028973
• Gene Symbol: MKKS
• Gene ID (NCBI): 8195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MKKS also known as BBS6 is a probable chaperone given to the amino acid similarity to the chaperonin family of proteins and may play a role in protein processing in limb, cardiac and reproductive system development. The mutations in BBS6 have been linked to Bardet-Biedl syndrome (BBS) which is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. It may also get involved in cellular organization processes, in particular relating to ciliary/flagellar and centrosomal activities.