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BBS6 Polyclonal antibody

BBS6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13078-1-AP

Synonyms

MKKS, MKS, McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin, KMS, HMCS

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using BBS6 Polyclonal antibody (13078-1-AP)

    WB analysis of mouse testis using 13078-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13078-1-AP (BBS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using BBS6 Polyclonal antibody (13078-1-AP)

    WB analysis of mouse brain using 13078-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13078-1-AP (BBS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BBS6 Polyclonal antibody (13078-1-AP)

    IHC staining of mouse testis using 13078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13078-1-AP (BBS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BBS6 Polyclonal antibody (13078-1-AP)

    IHC staining of mouse testis using 13078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13078-1-AP (BBS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS6 Polyclonal antibody (13078-1-AP)

    IF Staining of hTERT-RPE1 cells using 13078-1-AP

    IF result (cytoplasm and the base of cilia stain) of anti-BBS6 (13078-1-AP; 1:50) with hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

Tested Applications

Positive WB detected inmouse testis tissue, mouse brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13078-1-AP targets BBS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS6 fusion protein Ag3785
Full Name McKusick-Kaufman syndrome
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC028973
Gene Symbol MKKS
Gene ID (NCBI) 8195
RRIDAB_10603370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MKKS also known as BBS6 is a probable chaperone given to the amino acid similarity to the chaperonin family of proteins and may play a role in protein processing in limb, cardiac and reproductive system development. The mutations in BBS6 have been linked to Bardet-Biedl syndrome (BBS) which is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. It may also get involved in cellular organization processes, in particular relating to ciliary/flagellar and centrosomal activities.

Protocols

Product Specific Protocols
WB protocol for BBS6 antibody 13078-1-APDownload protocol
IHC protocol for BBS6 antibody 13078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Neurobiol

Inpp5e Regulated the Cilium-Related Genes Contributing to the Neural Tube Defects Under 5-Fluorouracil Exposure

Authors - Xiuwei Wang
View Article