BBS5 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 83722-1-RR (same clone as 83722-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83722-1-RR (BBS5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83722-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 83722-1-RR (same clone as 83722-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83722-1-RR (BBS5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83722-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 83722-1-RR (same clone as 83722-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using BBS5 antibody (83722-1-RR, Clone: 240674B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 83722-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00703-1

  Cytometric bead array standard curve of MP00703-1, BBS5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83722-2-PBS. Detection antibody: 83722-1-PBS. Standard: Ag6153. Range: 2.5-80 ng/mL.

BBS5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 83722-1-PBS

Clone No.240674B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

240674B5, Bardet Biedl syndrome 5, Bardet-Biedl syndrome 5 protein

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Product Information

83722-1-PBS targets BBS5 as part of a matched antibody pair:

MP00703-1: 83722-2-PBS capture and 83722-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BBS5 fusion protein Ag6153
• Full Name: Bardet-Biedl syndrome 5
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC044593
• Gene Symbol: BBS5
• Gene ID (NCBI): 129880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N3I7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.