BBS4 Recombinant antibody

• Featured Product
• KD/KO Validated

BBS4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230303C2

Cat no : 82961-1-RR

Synonyms

Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2

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Validation Data Gallery

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  WB analysis using 82961-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82961-1-RR (BBS4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82961-1-RR

  WB result of BBS4 antibody (82961-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-BBS4 transfected HEK-293 cells.

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  IF Staining of hTERT-RPE1 using 82961-1-RR

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS4 antibody (82961-1-RR, Clone: 230303C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

• 

  FC experiment of HeLa using 82961-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug BBS4 Recombinant antibody (82961-1-RR, Clone:230303C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82961-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82961-1-RR against Human BBS4 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, SH-SY5Y cells
• Positive IF/ICC detected in: hTERT-RPE1 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82961-1-RR targets BBS4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BBS4 fusion protein Ag3430
• Full Name: Bardet-Biedl syndrome 4
• Calculated Molecular Weight: 519 aa, 58 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC027624
• Gene Symbol: BBS4
• Gene ID (NCBI): 585
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).