BBS4 Recombinant antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

BBS4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230303C2

Cat no : 82961-1-PBS

Synonyms

Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2

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Validation Data Gallery

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  WB analysis using 82961-1-RR (same clone as 82961-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82961-1-RR (BBS4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82961-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 82961-1-RR (same clone as 82961-1-PBS)

  WB result of BBS4 antibody (82961-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-BBS4 transfected HEK-293 cells. This data was developed using the same antibody clone with 82961-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 82961-1-RR (same clone as 82961-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS4 antibody (82961-1-RR, Clone: 230303C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 82961-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82961-1-RR (same clone as 82961-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug BBS4 Recombinant antibody (82961-1-RR, Clone:230303C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82961-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00130-1

  Cytometric bead array standard curve ofMP00130-1, BBS4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82961-1-PBS. Detection antibody: 82961-3-PBS. Standard: Ag3430. Range: 0.313-20 ng/mL.

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  Affinity and Kinetic Characterization of 82961-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82961-1-RR against Human BBS4 were performed. The affinity constant is below 1 pM.

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Product Information

82961-1-PBS targets BBS4 as part of a matched antibody pair:

MP00130-1: 82961-1-PBS capture and 82961-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BBS4 fusion protein Ag3430
• Full Name: Bardet-Biedl syndrome 4
• Calculated Molecular Weight: 519 aa, 58 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC027624
• Gene Symbol: BBS4
• Gene ID (NCBI): 585
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).