CoraLite® Plus 488-conjugated BAZ2A Recombinant antibody

BAZ2A Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230205H8

Cat no : CL488-82910

Synonyms

BAZ2A, DKFZp781B109, FLJ13768, FLJ13780, FLJ45876, hWALp3, KIAA0314, TIP5, TTF I interacting protein 5, WALp3

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Validation Data Gallery

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  IF Staining of A549 using CL488-82910

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 BAZ2A antibody (CL488-82910, Clone: 230205H8 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82910 targets BAZ2A in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BAZ2A fusion protein Ag33963
• Full Name: bromodomain adjacent to zinc finger domain, 2A
• Calculated Molecular Weight: 211 kDa
• Observed Molecular Weight: 250-270 kDa
• GenBank Accession Number: NM_013449.4
• Gene Symbol: BAZ2A
• Gene ID (NCBI): 11176
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.