CoraLite® Plus 647-conjugated BAX Monoclonal antibody

BAX Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4G5E8

Cat no : CL647-60267

Synonyms

Apoptosis regulator BAX, BAX

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Validation Data Gallery

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  IF Staining of HeLa using CL647-60267

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 BAX antibody (CL647-60267, Clone: 4G5E8 ) at dilution of 1:100.

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  IF Staining of MCF-7 using CL647-60267

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 647 BAX antibody (CL647-60267, Clone: 4G5E8 ) at dilution of 1:200.

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  FC experiment of HeLa using CL647-60267

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human BAX (CL647-60267, Clone:4G5E8) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HeLa cells, MCF-7 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60267 targets BAX in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BAX fusion protein Ag21068
• Full Name: BCL2-associated X protein
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC014175
• Gene Symbol: BAX
• Gene ID (NCBI): 581
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).