BAT1 Monoclonal antibody, PBS Only

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  WB analysis using 67641-1-Ig (same clone as 67641-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67641-1-Ig (BAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67641-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 67641-1-Ig (same clone as 67641-1-PBS)

  IP result of anti-BAT1 (IP:67641-1-Ig, 4ug; Detection:67641-1-Ig 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 67641-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67641-1-Ig (same clone as 67641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67641-1-Ig (BAT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67641-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67641-1-Ig (same clone as 67641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67641-1-Ig (BAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67641-1-PBS in a different storage buffer formulation.

BAT1 Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 67641-1-PBS

Clone No.2G9H8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

DDX39B, UAP56, DDX39, D6S81E, ATP-dependent RNA helicase p47

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Product Information

67641-1-PBS targets BAT1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BAT1 fusion protein Ag6612
• Full Name: HLA-B associated transcript 1
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC000361
• Gene Symbol: BAT1
• Gene ID (NCBI): 7919
• RRID: AB_2882841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13838
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.