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BAT1 Monoclonal antibody

BAT1 Monoclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2G9H8

Cat no : 67641-1-Ig

Synonyms

DDX39B, UAP56, DDX39, D6S81E, ATP-dependent RNA helicase p47

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAT1 Monoclonal antibody (67641-1-Ig)

    WB analysis using 67641-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67641-1-Ig (BAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using BAT1 Monoclonal antibody (67641-1-Ig)

    IP experiment of HeLa using 67641-1-Ig

    IP result of anti-BAT1 (IP:67641-1-Ig, 4ug; Detection:67641-1-Ig 1:2000) with HeLa cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BAT1 Monoclonal antibody (67641-1-Ig)

    IHC staining of human breast cancer using 67641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67641-1-Ig (BAT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BAT1 Monoclonal antibody (67641-1-Ig)

    IHC staining of human breast cancer using 67641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67641-1-Ig (BAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67641-1-Ig targets BAT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BAT1 fusion protein Ag6612
Full Name HLA-B associated transcript 1
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC000361
Gene Symbol BAT1
Gene ID (NCBI) 7919
RRIDAB_2882841
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.

Protocols

Product Specific Protocols
WB protocol for BAT1 antibody 67641-1-IgDownload protocol
IHC protocol for BAT1 antibody 67641-1-IgDownload protocol
IP protocol for BAT1 antibody 67641-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols