CoraLite®594-conjugated BAG3 Polyclonal antibody

• Featured Product
• KD/KO Validated

BAG3 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10599

Synonyms

BAG 3, BAG3, BIS, CAIR 1, Docking protein CAIR 1

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Validation Data Gallery

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  IF Staining of A549 using CL594-10599

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 BAG3 antibody (CL594-10599) at dilution of 1:100.

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  IF Staining of A549 using CL594-10599

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 BAG3 antibody (CL594-10599) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10599 targets BAG3 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAG3 fusion protein Ag0956
• Full Name: BCL2-associated athanogene 3
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 74-80 kDa
• GenBank Accession Number: BC006418
• Gene Symbol: BAG3
• Gene ID (NCBI): 9531
• RRID: AB_2919741
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.