Filter: AllWBIPIHCIF-P

WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
WB analysis using 12807-1-AP (same clone as 12807-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
WB analysis of zebrafish using 12807-1-AP (same clone as 12807-1-PBS)
zebrafish tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 12807-1-AP (same clone as 12807-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
WB analysis of mouse pancreas using 12807-1-AP (same clone as 12807-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 12807-1-AP (same clone as 12807-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)
IP result of anti-BACE1 (IP:12807-1-AP, 3ug; Detection:12807-1-AP 1:500) with mouse brain tissue lysate 5000ug. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
IP experiment using 12807-1-AP (same clone as 12807-1-PBS)
IP result of BACE1 antibody (12807-1-AP for IP and Detection) with mouse brain tissue lysate. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12807-1-AP (same clone as 12807-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BACE1 antibody (12807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

BACE1 Polyclonal antibody, PBS Only

Name: BACE1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12807-1-PBS

BACE1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Cat No. 12807-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

ASP2, ASP21, Aspartyl protease 2, Beta secretase 1, Beta-secretase 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12807-1-PBS targets BACE1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3559 Product name: Recombinant human BACE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 157-457 aa of BC036084 Sequence: VRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLM Predict reactive species
Full Name	beta-site APP-cleaving enzyme 1
Calculated Molecular Weight	501 aa, 56 kDa
Observed Molecular Weight	70-75 kDa, 42-56 kDa
GenBank Accession Number	BC036084
Gene Symbol	BACE1
Gene ID (NCBI)	23621
RRID	AB_10638760
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P56817
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).