B2M Recombinant antibody, PBS Only (Capture)

B2M Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240660B1

Cat no : 83683-1-PBS

Synonyms

Beta-2-microglobulin, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2 microglobulin

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00673-3

  Sandwich ELISA standard curve of MP00673-3, human B2M Recombinant Matched Antibody Pair - PBS only. 83683-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4433. 83683-5-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

• 

  Sample test of MP00673-3

  Urine of eight individual healthy human donors was measured. The B2M concentration of detected samples was determined to be 202.46 ng/mL with a range of 67.46-352.77 ng/mL.

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  Sample test of MP00673-3

  Serum of eight individual healthy human donors was measured. The B2M concentration of detected samples was determined to be 223.48 ng/mL with a range of 111.44-289.89 ng/mL.

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  Cytometric bead array standard curve of MP00673-1

  Cytometric bead array standard curve of MP00673-1, B2M Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83683-1-PBS. Detection antibody: 83683-2-PBS. Standard: Ag4433. Range: 0.156-20 ng/mL.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83683-1-PBS targets B2M as part of a matched antibody pair:

MP00673-1: 83683-1-PBS capture and 83683-2-PBS detection (validated in Cytometric bead array)

MP00673-3: 83683-1-PBS capture and 83683-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: B2M fusion protein Ag4433
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.