B2M Monoclonal antibody, PBS Only (Detector)

B2M Monoclonal Antibody for Cytometric bead array, Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

4D4D5

Cat no : 68395-3-PBS

Synonyms

Beta-2-microglobulin, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2 microglobulin

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Validation Data Gallery

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  Sample test of MP50108-1

  Cytometric bead array of A375 cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of A375 cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

• 

  Sample test of MP50108-1

  Cytometric bead array of HeLa cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of HeLa cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

• 

  Sample test of MP50108-1

  Cytometric bead array of U-251 cells supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Gradient density (indicated at X axis) of U-251 cells were inoculated in 96 well plate followed by cultured for 2 days. The supernatant was analyzed using MP50108-1 (B2M Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the cell density （refer to bottom X - left Y axis). The left over cells were analyzed using cell proliferation assay reagent MTS. Signal was measured by absorbance at 492nm (refer to top X - right Y axis).

• 

  Sample test of MP50108-1

  Cytometric bead array of various cell lines supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Supernatant (50μL/well) from the following cell lines were assayed: LNCaP, THP-1, Karpas299, BT-549, HT-29, U266B1, Kasumi-1, Jurkat, HCT116, TT, MCF-7, K-562, Raji, NCCIT, BxPC-3, PC-3, Ramos, A549, SK-N-SH, U-251, HepG2, Caco-2, 293, 293T, hTERT-RPE1, A431, HT-1080, Saos-2, SH-SY5Y, UC-MSCS. Complete culture medium was served as blank control.

• 

  Sample test of MP50108-1

  Cytometric bead array of various cell lines supernatant using MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Supernatant (50μL/well) from the following cell lines were assayed: hTERT-RPE1, U251, A431, PC-12, ROS1728, NIH/3T3, mMSCS. Complete culture containing DMEM and FBS was served as blank control. Note that this matched antibody pair recognizes human B2M (beta 2 macroglobulin) but does not recognize rat or mouse B2M.

• 

  Cytometric bead array standard curve of MP50108-1

  Cytometric bead array standard curve of MP50108-1, B2M Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68395-2-PBS. Detection antibody: 68395-3-PBS. Standard:Eg31815. Range: 0.098-100 ng/mL.

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Product Information

68395-3-PBS targets B2M as part of a matched antibody pair:

MP50108-1: 68395-2-PBS capture and 68395-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.