CoraLite® Plus 488-conjugated Ataxin 2 Monoclonal antibody

Ataxin 2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E8A2

Cat no : CL488-68316

Synonyms

ataxin 2, ATX2, ATXN2, FLJ46772, SCA2, TNRC13

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Validation Data Gallery

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  IF Staining of rat brain using CL488-68316

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 Ataxin 2 antibody (CL488-68316, Clone: 1E8A2 ) at dilution of 1:200.

• 

  IF Staining of rat brain using CL488-68316

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 Ataxin 2 antibody (CL488-68316, Clone: 1E8A2 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68316 targets Ataxin 2 in IF-P applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ataxin 2 fusion protein Ag17402
• Full Name: ataxin 2
• Calculated Molecular Weight: 1313 aa, 140 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC114546
• Gene Symbol: ATXN2
• Gene ID (NCBI): 6311
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]