CoraLite® Plus 488-conjugated Apolipoprotein H Monoclonal antibody

Apolipoprotein H Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4D9A4

Cat no : CL488-66074

Synonyms

APOH, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein

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Validation Data Gallery

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  IF Staining of human liver cancer using CL488-66074

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 Apolipoprotein H antibody (CL488-66074, Clone: 4D9A4 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-66074

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Apolipoprotein H antibody (CL488-66074, Clone: 4D9A4 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66074 targets Apolipoprotein H in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Apolipoprotein H fusion protein Ag17915
• Full Name: apolipoprotein H (beta-2-glycoprotein I)
• Calculated Molecular Weight: 345 aa, 38 kDa
• Observed Molecular Weight: 50-53 kDa
• GenBank Accession Number: BC026283
• Gene Symbol: APOH
• Gene ID (NCBI): 350
• RRID: AB_2919283
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.