CoraLite® Plus 555-conjugated Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-14427

Synonyms

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1



Tested Applications

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-14427 targets Apolipoprotein AI in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag5793
Full Name apolipoprotein A-I
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC005380
Gene Symbol APOA1
Gene ID (NCBI) 335
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 Apolipoprotein AI antibody CL555-14427Download protocol
Standard Protocols
Click here to view our Standard Protocols