Apolipoprotein AI Monoclonal antibody, PBS Only

Apolipoprotein AI Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9G5

Cat no : 66206-1-PBS

Synonyms

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1

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Validation Data Gallery

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  WB analysis of human plasma using 66206-1-Ig (same clone as 66206-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66206-1-Ig (APOA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66206-1-Ig (same clone as 66206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66206-1-Ig (same clone as 66206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66206-1-Ig (same clone as 66206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66206-1-Ig (same clone as 66206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66206-1-Ig (same clone as 66206-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66206-1-Ig (APOA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66206-1-Ig (same clone as 66206-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Apolipoprotein AI (66206-1-Ig, Clone:1C9G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66206-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66206-1-PBS targets Apolipoprotein AI in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Apolipoprotein AI fusion protein Ag21920
• Full Name: apolipoprotein A-I
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: BC005380
• Gene Symbol: APOA1
• Gene ID (NCBI): 335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.