Apolipoprotein A II/APOA2 Monoclonal antibody, PBS Only

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  WB analysis of human plasma using 66773-1-Ig (same clone as 66773-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66773-1-Ig (Apolipoprotein A II/APOA2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66773-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66773-1-Ig (same clone as 66773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66773-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66773-1-Ig (same clone as 66773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66773-1-PBS in a different storage buffer formulation.

Apolipoprotein A II/APOA2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66773-1-PBS

Clone No.1G1A2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II

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Product Information

66773-1-PBS targets Apolipoprotein A II/APOA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Apolipoprotein A II/APOA2 fusion protein Ag9863
• Full Name: apolipoprotein A-II
• Calculated Molecular Weight: 100 aa, 11 kDa
• Observed Molecular Weight: 8-11 kDa
• GenBank Accession Number: BC005282
• Gene Symbol: APOA2
• Gene ID (NCBI): 336
• RRID: AB_2882119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02652
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)