Apolipoprotein A II/APOA2 Monoclonal antibody

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  WB analysis of human plasma using 66773-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66773-1-Ig (Apolipoprotein A II/APOA2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66773-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66773-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Apolipoprotein A II/APOA2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66773-1-Ig

Clone No.1G1A2

Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66773-1-Ig targets Apolipoprotein A II/APOA2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Apolipoprotein A II/APOA2 fusion protein Ag9863
• Full Name: apolipoprotein A-II
• Calculated Molecular Weight: 100 aa, 11 kDa
• Observed Molecular Weight: 8-11 kDa
• GenBank Accession Number: BC005282
• Gene Symbol: APOA2
• Gene ID (NCBI): 336
• RRID: AB_2882119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02652
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

Publications

Species	Application	Title
human	WB	Life Sci Alliance; SPHK1/S1PR1/PPAR-α axis restores TJs between uroepithelium providing new ideas for IC/BPS treatment; Authors - Junjie Zhang