Annexin VI Monoclonal antibody, PBS Only

Annexin VI Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H7G7

Cat no : 68086-1-PBS

Synonyms

Annexin A6, ANXA6, ANX6, Annexin-6, Annexin 6

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Validation Data Gallery

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  WB analysis using 68086-1-Ig (same clone as 68086-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68086-1-Ig (Annexin VI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68086-1-PBS in a different storage buffer formulation.

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  WB analysis using 68086-1-Ig (same clone as 68086-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68086-1-Ig (ANXA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68086-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68086-1-Ig (same clone as 68086-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Annexin VI antibody (68086-1-Ig, Clone: 2H7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68086-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 68086-1-Ig (same clone as 68086-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Annexin VI (68086-1-Ig, Clone:2H7G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68086-1-PBS in a different storage buffer formulation.

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Product Information

68086-1-PBS targets Annexin VI in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

• Tested Reactivity: rabbit, pig, rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin VI fusion protein Ag3263
• Full Name: annexin A6
• Calculated Molecular Weight: 673 aa, 76 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC017046
• Gene Symbol: Annexin VI
• Gene ID (NCBI): 309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.