Annexin A1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Annexin A1 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1B7

Cat no : 66344-1-PBS

Synonyms

Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1, p35

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Validation Data Gallery

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  WB analysis using 66344-1-Ig (same clone as 66344-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66344-1-Ig (same clone as 66344-1-PBS)

  WB result of Annexin A1 antibody (66344-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66344-1-Ig (same clone as 66344-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66344-1-Ig (same clone as 66344-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 66344-1-Ig (same clone as 66344-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66344-1-Ig (same clone as 66344-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 66344-1-Ig (same clone as 66344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 66344-1-Ig (same clone as 66344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66344-1-Ig (same clone as 66344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66344-1-Ig (same clone as 66344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66344-1-Ig (same clone as 66344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66344-1-Ig (same clone as 66344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 66344-1-Ig (same clone as 66344-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 66344-1-Ig(Annexin A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 66344-1-Ig (same clone as 66344-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug Anti-Human Annexin A1 (66344-1-Ig, Clone:1E1B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66344-1-PBS targets Annexin A1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin A1 fusion protein Ag17273
• Full Name: annexin A1
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC001275
• Gene Symbol: ANXA1
• Gene ID (NCBI): 301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.