CoraLite® Plus 647-conjugated Amphiregulin Polyclonal antibody

• Featured Product
• KD/KO Validated

Amphiregulin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-16036

Synonyms

amphiregulin, AR, AREG, AREGB, CRDGF, SDGF

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Validation Data Gallery

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  IF Staining of human colon cancer using CL647-16036

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 647 Amphiregulin antibody (CL647-16036) at dilution of 1:200.

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  IF Staining of human colon cancer using CL647-16036

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 647 Amphiregulin antibody (CL647-16036) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-16036 targets Amphiregulin in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Amphiregulin fusion protein Ag8907
• Full Name: amphiregulin
• Calculated Molecular Weight: 252 aa, 28 kDa
• GenBank Accession Number: BC009799
• Gene Symbol: Amphiregulin/AREG
• Gene ID (NCBI): 374
• RRID: AB_2920229
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).